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In order to avoid pseudogene amplification, Pou5f1 expression was additionally analyzed using the primer Mm00658129_gH Pou5f1 TaqMan gene expression assay (Applied Biosystems) and the TaqMan Fast Universal PCR Master Mix Applied Biosystemss), following manufacturer's instructions.
COX-2 expression was additionally analyzed by qRT-PCR using the following primers: forward: 5′-ATCATAAGCAGGGCCAGCT-3′, reverse: 5′-AAGGCGCAGTTTACGCTGTC-3′, resulting in a 101 bp fragment.
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The expression of EGFP was additionally analyzed by SDS-PAGE and Western blot using anti-GFP antibody.
The chromosomal distribution of genes with sex-biased expression in the male and female germlines was additionally analyzed.
Liver tissue with metastases was additionally analyzed for cytokines, T cell markers and Fas/Fas-L expression using immunohistochemistry, immunofluorescence and RT-PCR.
Previously published normal fetal brain regional expression data were additionally analyzed [ 41].
For GSE5460, raw expression values obtained from Affymetrix GENECHIP software were additionally analyzed using DNA-Chip analyzer (dChip) custom software (www.dchip.org).
Seven CNMS (CTT n/(GAA)n associated genes were additionally analyzed for expression patterns after salicylic acid treatment.
Furthermore, 14 samples were additionally analyzed by aCGH (BlueGnome, UK).
The tripartite motif domains were additionally analyzed as described below.
During labor, PROKR1 expression is additionally localized to the vasculature.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com