Exact(12)
Resistant cells were expanded and FLAG-tagged protein expression verified by western blot.
Individual clones were picked and gene expression verified by induction with 1 µg/ml doxycycline for 24 hours and anti-myc western blotting.
Cells with low to moderate GFP-AR expression were then single cell cloned and expression verified by western blot; subsequently, GFP-AR expression was found similar to or below the endogenous expression of AR in the LNCaP prostate cancer cell line [15].
* Expression verified by 454 transcriptome sequencing, **BLB = MHC Class IIB genes, *** BF = MHC Class I genes.
A miRNA microarray was performed on the three cell phenotypes and expression verified by qRT-PCR.
Quantification of vimentin expression verified that the cultured cells still had the ability to differentiate.
Similar(48)
Both lung resident macrophages and metastasis recruited macrophages also have similar Mac3 expression verifying the IHC staining (Figure S5).
All constructs showed GUS expression, verifying that the PvUbi1 and PvUbi2 promoters can be used successfully to drive transgene expression.
The functional differences in MEC subpopulations identifiable by MMTVrtTA/H2BGFP expression were verified by gene expression analysis.
The whole population of transfected cells was used, and Ras over-expression was verified by GFP expression (see "Results").
The theoretical expression is verified in our laboratory and can be utilized to correct the conventional temperature response expression.
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