Sentence examples for expression validation from inspiring English sources

Although these procedures have predicted many candidates with the potential to form qualified hairpins, only a limited number of hairpins have been confirmed experimentally on the basis of expression due to the difficulty of large-scale expression validation and low prediction accuracy for expressed miRNAs.

It is important to note that while we have successfully identified PF-4 as a biomarker of dietary response by differential expression, validation and isolation, univariate data analysis of the complete proteome validated an additional 9, differentially expressed peptides (p < 0.01) between these two groups of mice (Table 1).

Due to limitation in availability of RNA required for gene expression validation, the following genes were selected based on statistical significance of differentially expressed genes and their biological relevance: XIST, CREBZF, CCNL2, AHSA2, CIRBP, PILRB, OXTR, TNMD and SOX6 (Table  3).

The measurement and the model are generally linked through the approaches of analysis, expression, validation, and prediction.

For gene expression validation, 2 PCR primer pairs (Table 2) for each of the six comparisons were designed using Primer3 shareware (http://frodo.wi.mit.edu/cgi-bin/primer3/primer3_www.cgi).

This high throughput method has increased sensitivity and accuracy relative to microarrays, and demonstrates a 100% miRNA expression validation rate against standard qRT-PCR [6].

AB carried out screening procedures, conducted all expression validations, performed gene ontology annotations and helped to draft the manuscript.

For gene expression validations, RNA samples used for cDNA synthesis were DNase treated (Promega, Madison, WI) and reverse transcribed using Superscript III reverse transcriptase (Invitrogen, Carlsbad, CA), according to the manufacturer's instructions.

The results of the gene co-expression validation based on PGSC data are shown in Figure  6.

For the over-expression validation experiment, 6×10 HEK293FT cells transfected with 1μg of miR-30ac2 over-expression plasmid, using 3μl of FuGENE® transfection reagent (Promega).

Finally, we introduced an integrated annotation of the different pipelines that outperformed the single pipelines both in the gold standard validation and in the co-expression validation.