Hence, we wonder whether in SY5Y-APP cells, that expressed unfolded p53, the expression of GAP-43 was compromised.
To examine the expression of unfolded protein response (UPR) genes, a set of genes that are activated to assist in protein trafficking and cellular homeostasis when endoplasmic reticulum (ER) stress occurs, in inflamed and uninflamed periodontal tissues, with or without Russell bodies (RB).
It has previously been shown that newly differentiated cardiomyocytes from cardiac progenitors cells can be identified by their expression of unfolded DsRed protein in their cytoplasm [26], [27].
The expression of unfolded p53 (positiveositive) was significantly higher in SY5Y-APP clone than levels found in mock cells.
Slow-clearing parasites exhibited increased expression of unfolded protein response pathways (e.g., chaperone complexes); these pathways may mitigate protein damage caused by artemisinin.
113 The transcriptomes of resistant parasites showed increased expression of unfolded protein response pathways and prolonged ring-stage development, offering insights into the mechanism of artemisinin resistance.
Resistance to artemisinin is also associated with increased constitutive expression of unfolded protein response pathways, which are believed to mitigate toxicity of artemisinin (24 ).
However, when HEK-APP cells were transfected with CHIP, the folded conformation (PAb 1620) of p53 was restored and at the same time, a decrease in the expression of unfolded p53 was observed.
On the contrary, in SY5Y-APP cells the lack of p53 pro-apoptotic properties was the result of the presence of high expression of unfolded isoform that stabilize and rendered unfunctional also the wt quote.
In particular, 24 h 100 μℳ ZnCl2, a concentration that did not affect APP levels and processing (data not shown), reduced the expression of unfolded p53; notably there is also a decrease of p53wt levels, after zinc supplementation.
