In contrast, processes mediated by small RNAs appeared prevalent as a subset of co-expressed categories in profile 3, with an expression trend that increased towards late embryogenesis.
In contrast, GBSSI exhibited an up-down expression trend (Type II) and was mainly expressed at the intermediate stages of growth in wheat and Ae. peregrina.
PHE1 is preferentially expressed in endosperm from the paternal allele only, and therefore its expression trend in our data was as expected.
Further, a recent in planta microarray analysis in our lab revealed expression trend differences between UW551 and GMI1000.
As shown in Fig. 7A, phosphorylated Akt and Akt protein kinase showed an increased expression trend during increased PDs in response to GR.
One representative case of IHC data showing the predominant expression trend (up or down) for each candidate protein is shown in Figure 4B, upper panel.
The expression trend between miRNAs and their host genes is highly correlated for both delayed implantation and activation, suggesting that host genes and miRNAs are co-transcribed.
In the case of VWA5A, the expression trend is similar in MEFs and MCF7 cells but the difference in expression levels between the WT and MTA1 knockdown in the MCF7 cell line is marginal.
The immunoblotting data and the densitometry values of the protein bands as well as the predominant expression trend of the proteins across the MCF10AT model are shown in Figure 4A, left panel.
Western blot analysis in panel Fig. 6B confirmed the expression trend of MTA1 after castration at the protein level, with the exception of an undetected increase at postoperative C7+5 as demonstrated in RT-PCR assay.
The expression trend of their precursors was very similar to that of their corresponding miRNAs, except for pre-miR-6, which was significantly up-regulated under activation (Fig. 5C).
