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Considering the peak expression time for each diurnally regulated gene, and its corresponding functional assignment, most gene functions display temporal enrichment in the day, often with distinct patterns, such as dawn or midday preferred, indicating that there is a staged procession of biological events undulating with the diurnal cycle.
In soybean cotyledons, initial attempts to visualize GFP at the 24 hour time point, which is the peak expression time for the lima bean target [ 10], were unsuccessful as only very low levels of GFP were observed.
This hypothesis is supported by the expression data available for the DMSO and 2-BMP treated palmitomes, with the peak expression time for palmitoyl proteins affected by 2-BMP being skewed toward a later time point.
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The expression times for the transient assays were short and the protein expressed in leaves of N. benthamiana was still functional more than 5 d after transformation.
Figure 1 illustrates peak expression times for genes with reported or suspected involvement in insecticide metabolism and resistance.
We calculated asexual and sexual peak expression times for individual genes and, in their aggregate, overall for each cluster.
We generated 20 wild-type expression time series for each network as the basis for model estimation (see Figure 1F).
To further evaluate the degree of microRNA temporal expression conservation, we generated expression time courses for each pair of orthologs using the scaled developmental intervals, and calculated the resulting Pearson's correlation.
Emotional self-care: removing culpability, emotional expression, time management, asking for help, social networks and search for support.
However, wild-type experiments give expression time series data for a large number of genes at once.
Therefore, a lot of time and effort has been spent on developing tools that suit the need for expression time series analysis.
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CEO of Professional Science Editing for Scientists @ prosciediting.com