Sentence examples for expression tag from inspiring English sources

The protein was expressed with an insoluble expression tag (KSI tag) at the N-terminus in order to protect the peptide from proteolytic degradation in vivo.

It contains a complete reference genome sequence, Expressed Sequence Tags (ESTs), Massively Parallel Signature Sequencing (MPSS) expression tag data and related information from both public and private repositories.

To remove the expression tag, purified DHQD was incubated overnight at 4 °C with hexahistidine-tagged TEV protease and repurified by Ni-NTA chromatography.

To identify differentially expressed genes from H. armigera and H. assulta, digital gene expression tag (DGE-tag) profile libraries were constructed and sequenced using high throughput second-generation sequencing technology [ 12, 13].

The His ZZ expression tag was completely cleaved off by adding 1 100 (w/w ratio) His-tagged TEV (tobacco etch virus) protease directly to the dilute eluate from the affinity column and left to incubate for 12 h at 4°C.

The technology developed by Illumina (formerly Solexa sequencing) [ 12], also referred to as Digital Gene Expression tag profiling (DGE) [ 13], allows millions of short RNAs and differentially expressed genes to be identified in a sample without the need for prior annotations.

Conversely, most of the statistical tests currently used to detect differentially expressed genes are based on asymptotic results, and perform poorly for low expression tags.

A small number of rationally designed short expression tags is attached via overlap PCR to the 5-prime end of the target protein coding sequence.

The expression tags can be minimized to only a few codons and no further impact on the coding sequence is required.

At first, we selected 47 larval stage high expression tags to determine their expression levels by qRT-PCR.

Only those tags expressed in at least two samples at detectable levels (≥1 TPM) were defined as high-confidence novel mRNA expression tags.