The relevant genes and clusters were supported by the co-expression network structure, functional enrichment of co-expressed genes, gene expression specificity and literature information.
Expression specificity of modules is expressed as the percentage of module members specifically expressed in a particular tissue/condition (and across all arrays) with an expression specificity index above 1.
Additionally, the highly interactive expression specificity chart showed that the grapevine VvCEB1 gene was expressed specifically in berry-related tissues with highest specificity during veraison onwards.
A gene was considered well and specifically expressed in the corresponding experimental condition when the probeset expression specificity index values were > 1 and > 5, respectively.
Similarly, the cluster cumulative expression specificity index (cESI) was defined as the fraction of cluster members specifically expressed in a particular tissue or condition (and across all arrays) with an expression specificity index above 1, according to [ 9].
These studies validate the use of tissue-specific silencer elements to promote enhanced transgene expression specificity.
As might be expected from their frequent narrow expression specificity, lincRNA loci are substantially depleted in broadly expressed euchromatin ("yellow", fig. 3 C ) and in HP1-associated heterochromatin ("green", fig. 3 C ).
According to the expression specificity analysis results, 14 of 201 unigenes were specially expressed in the 3-5 DPA caryopsis.
Inspection of the cluster expression specificity index showed that a large fraction of genes (>70%) was specifically expressed in fruit peels (flavedo) of sweet oranges and grapefruit, but to a lesser extent in whole fruits of lemon (>50%) and with low expression specificity in leaf, flower and root tissues.
We have followed up with new protein sequencing experiments where the expression specificity of the LCNEC phenotype can be identified, as shown in Figure 2.
More importantly, the combined use of AGRO100 and PEG enhanced tumor gene expression specificity and effectively reduced toxicity in reticuloendothelial system (RES) organs after intravenous injection.
