Purified RNA was sent to NimbleGen Systems, Inc. (Madison, WI) for full expression services.
We thank Linda Manley for technical assistance with the laboratory animals, Linda Solfjell for molecular bioengineering, Aaste Aursjø for immunohistochemistry handcraft, Anne Kilsund Axelssen for all bacteriological work, and the FUGE funded Microarray Core Facility for providing gene expression services.
High-density microarray analyses of gene expression were carried out with week 15 RNA samples (n = 3 for each diet groups) [20] by the Affymetrix Gene Expression Service Lab of Academia Sinica.
We thank member of gene expression service at The Jackson Laboratory, especially Sonya Kamdar for technical support, and Dr. Shannon McWeeney at OHSU for helpful discussion on study design and data analysis.
Two-day EBs were treated with or without 100 ng/ml FGF10 and RNA was obtained 24 hours later for microarray analysis, which were performed by the Affymetrix Gene Expression Service Lab (http://ipmb.sinica.edu.tw/affy/), supported by the Academia Sinica.
Microarray service was provided by Phalanx Biotech (OneArray Gene Expression Service).
Total RNA of 1 μg from each sample was shipped to Asuragen Services for miRNA profiling via their DiscovArray miRNA Expression Service (Asuragen Services; Austin, TX, USA).
The Affymetrix GeneChip assays were performed by the Affymetrix Gene Expression Service Lab (http://ipmb.sinica.edu.tw/affy/), supported by Academia Sinica, Taiwan.
The quality control of RNA, preparation of cRNA and hybridization of oligonucleotide chips was performed by the Affymetrix Gene Expression Service Lab. at the IPMB, Academia Sinica.
The right cortex and remaining parts of the right brain, herein called the midbrain hindbrain, were isolated from each other for RNA extraction, cDNA preparation and Affymetrix microarray processing done in The Jackson Laboratory Gene Expression service.
RNA samples from two biological repeats were sent to the Affymetrix Gene Expression Service Lab at Academia Sinica, Taipei, Taiwan (http://ipmb.sinica.edu.tw/affy/) for target preparation, and hybridization to the GeneChip Rice Genome Array (Affymetrix, Santa Clara, CA, USA).
