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In a previous test for a regulatory role of antisense RNA expression, sense and antisense transcripts of PYR3, a dihydroorotase, were expressed in U. maydis.
Fluorescently tagged siRNA duplexes were designed to knockdown human CRMP4 (both long "L" and short "S" isoforms) protein expression: sense 5' Alexa 488 GUG UUG AUG ACG UAC GUU ATT 3', antisense 5' UAA CGU ACG UCA UCA ACA CTT 3' (Invitrogen).
Equal cDNA amounts from each sample were amplified using the following primers to detect PLK1 expression: sense, 5'- TTCGTGTTCGTGGTGTTGGA-3'; reverse, 5'- CTCGTCATTAAGCAGCTCGT-3' (563 bp).
These results lead to the key observation that it isn't the overall level of expression (sense antisense) that is critical but rather it is the fact that expression comes from multicopy genes (rather than one highly expressed gene).
Although the viruses are different, once they enter the host cells they all must reprogram cellular gene expression, sense cell-cycle phase, modify cell-cycle progression and reactivate the lytic life cycle to produce new virions to spread infection [37].
A 22 nt anti-GFP siRNA sequence identified previously [ 32] as an effective inhibitor of pd1EGFP expression (sense strand: 5'-UUG UGG CCG UUU ACG UCG CCG U-3', antisense strand: 3'-UGA ACA CCG GCA AAU GCA GCG G-5') was utilized in this study.
Similar(54)
That is, unless you count freedom of expression, common sense, and humor.
Alterations in galectin-3 levels by expression of sense or antisense galectin-3 constructs resulted in parallel alterations of MUC2 protein and RNA.
Let us assume that A is the long-established sign (expression) whose sense we have attempted to analyse logically by constructing a complex expression that gives the analysis.
Eyes shine, the voice gains in expression, a sense of excitement and of being alive exists.
One possible explanation is that the expression of sense transcripts (protein-coding) is inhibited by antisense RNAs under normal growth condition.
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