Altogether these data strongly suggest that the natural and progressive decrease of SRF could contribute to some of the age-related muscle alterations and that our model of inducible SRF down expression reproduce an accelerated aging process.
Our genetic analysis in eye disc clones indicates that, although Crb over-expression reproduces many of the scrib mutant defects, the scrib phenotype is not dependent upon Crb activity.
These data indicate that while Crb over-expression reproduces many of the scrib mutant defects, ectopic Crb activity is not responsible for the scrib mutant phenotype and, therefore, Crb is likely to function either upstream or independently from Scrib in the larval eye disc.
This expression is reproduced from Gerardi et al. [22] in order to reinforce the concept that the contribution of each nucleation site to the partitioned heat fluxes is accounted for.
We verified that pooled miRNA expression results reproduced the average of single subject values, though as modeled in Peng et al [21], this likely represents a group difference (Supplementary Figure S3).
As visualized by IF-staining, the G-2 cell pattern of cytokeratin expression was reproduced in G-2 cell derived clones (Figure S1A B; shown as example for clones G-2C9 and G-2C11), although according to qPCR analysis the relative levels of Krt14 and Krt18 gene expression varied markedly between clones (Figure S1C D).
The time-of-day-dependent variation in JCP-induced basophil CD203 expression was reproduced on different days in individual SAR subjects (Fig. S2 and data not shown).
This pattern of MMR expression was reproduced by synovial fibroblasts exposed to reactive nitrate species and to a smaller extent by fibroblasts exposed to ROS, indicating a role for oxidative stress in the development of microsatellite instability in RA.
When we considered genes with at least ten reads in each condition, ninety-five percent of gene expression measurements reproduced within a 1.8x fold-change between the two technical replicates.
While a single copy of POS9 did not produce detectable expression, when this element was present in combination with either the 5′ (termed POSX) or 3′ (termed POSY) P-INO flanking regions, or at least three additional copies of POS9, the wild-type pattern of INO expression was reproduced.
