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This points out, that the lower concentration of inclusion bodies simply results from lower expression rates of the d-Hyd(co) instead of improved protein folding.
To do this, we used two different cell lines characterized by different expression rates of the insulin receptor.
In order to determine the expression rates of the selected genes, all PCR assays were performed in triplicate.
Thus, we studied expression rates of the potential target sequences hTERT, CK 19, and onfFN in PTCs, FTCs, nonmalignant thyroid tissue, as well as in leukocyte subgroups.
The G-660 allele is linked to the lowest expression rates of the HSP90AA1 gene under both heat stress and mild temperature conditions.
The initial expression rates of the TFs in each cell were assigned from a normal distribution with μ = 1.5 and sd = 1.
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Furthermore, for simplicity, the basal expression rates of these three genes are assumed to be zero.
The gene was constructed taking into account the codon preference of Escherichia coli (CAI value 0.99) in order to increase the expression rate of the DNA in the host strain.
The most important role in implementing a tumorigenesis is played by an expression rate of the molecule.
In general, fully evaluated protocols able to deliver reproducible and comparable results are needed to determine the true range of variation of Bt concentration in the plants and the expression rate of the newly introduced proteins.
These results indicate that some proteins, in most cases probably low-abundance proteins, are still missing in our protein inventory of cells because of the low expression rate of the corresponding genes.
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