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The differentially expressed miRNAs between the two libraries were showed in Figure 3 and Additional file 4. The same as previous study on miRNA detecting [ 10, 11, 13, 27, 35, 36], most of its expression quantity were equivalent, while there were also some miRNAs expressed differently between the two experimental group.
To examine the relationships between gene expression and gene structure for Arabidopsis and rice genes, I firstly calculated Spearman's rank sum correlation coefficients between various structural parameters and total expression level (Exp tot, the sum of expression quantity of a gene acrosss tissues where the gene is expressed).
Another expression parameter, average expression level (Exp avg, average expression quantity of a gene across tissues in which the gene is expressed), shows a weaker correlation with expression breadth (Spearman's ρ = 0.70 or 0.81, for Arabidopsis or rice genes).
The Y-axes indicate the relative expression quantity.
MIDAS utilizes gene expression quantity information fully and the network centrality information to determine condition specific subpaths.
The largest relative expression quantity was nearly 2-fold at day 5 in DN422 and 2.5-fold at day 7 in KY131.
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As requested by the software, Ct values were converted to linear expression quantities by 2-ΔCt using the highest expressed sample as calibrator.
However, the Cx-43 expression quantities in the three groups showed no significant difference.
In temporal patterns, expression quantities peaked from 6 to 8 h after bacterial infection, and tended to decrease later (Fig. 4A and 4C).
Protein expression quantities were determined by IOD.
The expression quantities of cyclin D1 protein in aorta cells were detected by fluorescence immunoassay.
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