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Efforts to this end are hindered by the lack of suitable cultured stromal cell models, especially ones that express AR at sufficient levels to allow the use of contemporary mass gene expression profiling techniques.
Expression profiling techniques have been used to simultaneously monitor the expression of thousands genes from biological samples.
We have conducted a correlation analysis to determine the agreement in the estimated gene expression fold changes (log2 thereof) obtained with high-throughput expression profiling techniques and qRT-PCR.
Herein, we benchmark three state-of-the-art expression profiling techniques to assess in maternal circulation the expression of cell type-specific gene sets previously discovered by single-cell genomics studies of the placenta.
© 2015 Elsevier Inc. High-throughput expression profiling techniques bring novel tools and also statistical challenges to genetic research.
Current gene expression profiling techniques helps us in predicting the transcription factors conferring to β cell regeneration[13].
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A recently developed expression profiling technique, termed comparative expressed sequence hybridisation (CESH), was applied for the study of lymph-node negative breast cancer.
SAGE is an expression profiling technique that simultaneously measures the levels of thousands of genes expressed in a given tissue.
Therefore, the goal of this work was to compare Affymetrix Human Transcriptome Arrays (HTA 2.0) that probe the transcriptome at the exon level, paired-end Illumina RNA-Seq with globin reduction, and a novel genome-wide targeted expression profiling technique DriverMap (https://www.cellecta.com).
Targets of these novel miRNAs were determined by in silico prediction while their negatively correlated expression measured using MACE, a high-throughput expression profiling technique.
Further, the reproducibility of data from microarray is questionable in spite of the fact that this is the most common expression profiling technique used [ 5- 9].
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