Sentence examples for expression preparation from inspiring English sources

There is a detailed analysis of methods designed to increase the number of available samples for structural analysis via novel expression, preparation, and optimization systems.

Details of the expression, preparation, kinetic analyses, and thermal denaturation assays are described in Supporting Information.

Microarray gene expression preparation and processing procedure were done as recommended by the manufacturer (Affymetrix, Inc .. Raw expression values were extracted and normalised by the Affymetrix Power Tools based on Robust Multiarray Average (RMA) approach.

Tag libraries from the RNA samples were prepared in parallel using an Illumina gene expression sample preparation kit and sequenced using the Illumina GAII platform at BGI-Shenzhen (http://en.genomics.cn/navigation/index.action) [ 50].

In a study of liver gene expression in preparation, fatty acid desaturases SCD and FADS1 were significantly downregulated in liver, where we identified a multifunctional protein TOB1 which is significantly upregulated.

Expression cassette preparation.

Bacterial expression and preparation of GST-fusion proteins was performed according to standard procedures.

Heterologous expression and preparation of the recombinant protein was carried out according to [ 35].

Expression vector preparation: 1. Isolate at least 300 μg of plasmid DNA using a commercial midiprep kit (e.g., GenElute HP Plasmid Midiprep Kit [Sigma-Aldrich, St .Louis, Missouri, USA] or Nucleobond Xtra Midi [Machery-Nagel, Düren, Germany]) or another method.

(B ) The indicated human CD4+ T-cell subpopulations were FACS sorted based on CD3, CD4, CD45RO, and CD25 expression from preparations of peripheral blood mononuclear cells (PBMCs) from healthy human donors.

Expression plasmids: The preparation of plasmid constructs expressing PPARα, γ and δ has been described previously.