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To check GST-protein expression, a portion of GST-protein-conjugated beads was denatured in 4× LDS (Life Technologies, NP0007) at 70°C for 15 min and subjected to SDS-PAGE followed by Coomassie Blue staining (Invitrogen, LC6060).
To control for aberrant splicing or the presence of an internal promoter in the utrophin A 5'UTR, qRT-PCR control experiments were conducted as previously described to detect expression of portions of βGAL and CAT from the bicistronic mRNA transcript [16], [43], [56].
Many expressions, many portions of sentences are individually invented for the occasion, or at least so it seems.
At the same time, considerable progress has been made in the detailed description of the subcellular-, cellular-, tissue- and organ-specific expressions of portions of the biosynthetic pathways leading to the production of MIAs, revealing a complex picture of the transport of biosynthetic intermediates among membrane compartments, cells and tissues.
This showed that ageing of animals from 14 weeks (at the beginning of the experiment) to 30 weeks (at its end) affected expression of a portion of genes, also in the control condition.
Manipulating competition rules in models could be used to check the intuition that reducing the expression in a portion of the SC might be expected to magnify the map from the retina in this region.
In a first attempt to test its expression an internal portion of cDNA synthesized from mRNA of a paraquat-induced M. grisea culture was amplified using Cloned AMV First Strand cDNA Synthesis Kit (Invitrogen).
Inhibition also diminishes the number of blood vessels in the prostate because of a reduction in vascular-derived endothelial growth factor which downregulates the eNOS expression in lateral portion of prostate [ 27].
The same antibody, here, employed to stain a wide range of normal, dysplastic and neoplastic tissues yielded a pattern similar to that of Ki-67 (Spearman R = 0.88; p < 0,001), underlining the close association between cell proliferation and the expression of that portion of p27 targeted for degradation.
In our data (Table 6), most FL-AE appeared as consistently-highly expressed genes, while expression of a large portion of CDS-ANE was consistently low.
