Briefly, prokaryotic vector E. coli BL21 (DE3) strain containing the expression plasmid was cultured and induced to express HBc VLPs.
This yielded the expression plasmid pVK51dkr.
The expression plasmid was built in two steps.
These ribozymes were cloned in a mammalian expression plasmid, pZeoSV2.
The mammalian expression plasmid for GFP-EB1 and the bacterial expression plasmid for GST-EB1 were constructed by cloning EB1 cDNA into the pEGFPN1 and pGEX6P3 vectors, respectively.
Moreover DNA vaccination with the IL-2 expression plasmid induced a Th1 dominant immune response.
Fig. 2 Construction of the food-grade expression plasmid pMA5-DAL-RDPE.
The shRNA expression plasmid, pmbv43, has been previously described (Buttaro and Fruehauf 2010).
Flp expression plasmid was introduced into the HUES3 KI cells by electroporation.
These constructs were co-transfected into LM2 cells with or without a JUN expression plasmid.
This is the first report of an expression plasmid containing a Rps.
