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A great expression, period".
32 factorial design was used to appraise the influence of temperature and inducer concentration on enzyme expression every hour for the 4-h expression period.
Following an expression period of 14 h at 25°C and the subsequent IMAC purification, 2.5 mg of Tcur1278 and 2.9 mg of Tcur0390 were obtained from a 500 mL culture with a specific activity of 3.0 U/mg and 17.9 U/mg against pNPB, respectively.
Investigation of the ohyA expression and biotransformation conditions (e.g., inducer concentration, gene expression period before initiating biotransformation, mixing condition of reaction medium) enabled 10-hydroxystearic acid to accumulate to a final concentration of approximately 46 g/L in the culture medium.
For expression of HSL, cell cultures (2×106 cells/mL) were infected at a multiplicity of infection of 10 followed by a 72 h expression period.
During the expression period, the number of cells observed in the DX culture did not differ significantly from the tetracycline control even though the tetracycline culture had a lower number of CFUs at each time point.
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The species with long expression periods in the Boolean oscillation (Tcr bind, PAG Csk, Fyn, TCR phos and IkB) were expressed at high or medium levels whereas the species with short expression periods were not expressed at all.
In this case, shorter expression periods were observed in association with phalangeal and digit reduced taxa [ 18].
The mammalian target of rapamycin (mTOR) canonical pathway was significantly altered during early (p = 2.16E-05) and mid (p = 4.79E-02) experiodsn periofs of liver growth.
In order to reflect the different gene reorganization events happening on time points t2 and t3, these genes are divided into two temporal blocks during the co-expression period.
PKA expression rescues period defects induced by shits1, suggesting a functional link between the membrane, PKA, and behavioral period.
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Justyna Jupowicz-Kozak
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