Exact(11)
Interestingly, the expression partitioning of homeologs exhibited temporal or stress-specific patterns (Fig. 6b).
The absence of gene expression partitioning among the apple PSYs contrasts with what is observed in plants, such as maize, where subfunctionalization is observed among the PSYs [ 20].
The reunion of two diverged genomes in a common nucleus during allopolyploid speciation entails a suite of genomic accommodations [ 12- 14], including non-additivity of gene expression [ 15, 16], and expression partitioning among tissues and organs [ 17- 20].
Finally, approximately 68.4 % of homeologous genes were found to exhibit expression partitioning in response to DS, HS or HD, which was further confirmed by using quantitative RT-PCR and Nullisomic-Tetrasomic lines.
After finding intact and expressed numit rps13 and mt rps13 genes in Malus we tested the hypothesis that there has been expression partitioning of the two genes in different organ types and/or stress conditions to preserve both genes.
Nullisomic-Tetrasomic line detection indicated our primers were homeolog specific and qRT-PCR results showed their expression partitioning was consistent with our observation obtained from RNA-seq data (Additional file 16, Fig. 7).
Similar(49)
Clusters generated by global expression profiles partitioned samples in well-distinguished subgroups that overlapped with clusters obtained using "biologic scores" (cytohistologic variables) and differed from clusters based on "technical scores" (RNA/complementary RNA/microarray quality).
Gene expression was partitioned into four categories: no, low (log2‐scaled averages <4), medium (log2‐scaled averages ≥4 and <8) and high expression (log2‐scaled averages ≥8).
The genes with significant change in expression are partitioned into three groups based on the time of their highest fold change in expression - initial response genes, intermediate regulators and late effectors.
In summary, our work contributes a novel approach to the problem of partitioning expression samples and detecting potential modulators of expression correlation between a pair of genes.
Since PIN proteins show a definite spatial-temporal expression and there partitioning in the cell is very important for determining the flow of auxin transport and thus the gravitropism, we also checked the expression of PIN2 GFP [10] proteins.
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