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We normalized the expression of the target genes using the constitutively expressed gene hypoxanthine guanine phosphoribosyltranferase (HPRT).
Therefore, structural determination of most eukaryotic IMPs requires recombinant expression of the target proteins.
The relative expression of the target genes was normalized to beta-actin expression.
The relative expression of the target genes was measured using the comparative Ct method (Livak and Schmittgen 2001).
More importantly, the expression of the target gene would also be coupled with the expression of the reporter mCherry.
A confocal microscope (LSM510; Carl Zeiss; Germany) was used to analyse the expression of the target protein [24].
A mathematical expression of the target failure rate for overpressure of the twin stream subsystem is presented and analyzed.
A prerequisite for success in protein purification is sufficient soluble protein expression of the target protein in a heterologous host.
Expression at 0 h was used as the calibrator (designated as 1.0) to determine the relative expression of the target gene at different time points.
The relative gene expression of the target gene was calculated using the equation: Exp = 2-ΔCt, where ΔCt = Cttargetgene − Ct eEF1a (Livak and Schmittgen 2001).
The uniform expression of the target glycan in the cell set is undesirable.
More suggestions(18)
expression of the history
expression of the narrator
expression of the harvest
expression of the war
expression of the king
expression of the distance
expression of the world
expression of the technology
expression of the meaning
expression of the belief
expression of the demand
expression of the blogger
expression of the knowledge
expression of the neighborhood
expression of the life
expression of the play
expression of the campaign
expression of the baritone
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