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We applied in situ hybridization for measuring changes in the visually driven expression of the activity reporter gene zif268, to visualize possible differences in activation of the central and peripheral region of primary visual cortices 17 and 18 as a function of age and visual manipulation.
Previously, we developed a quadruplex hammerhead ribozyme (QHR) whose conformational change, from a duplex to a G-quadruplex, triggered by K+ results in expression of the activity.
Introduction of the appropriate substituent into the 2-position of the adenine nucleus gave rise to expression of the activity even in 9-benzyladenines bearing no hydroxyl group at the 8-position.
To better understand the neural systems underlying these classification tasks, we examined neural activity patterns in response to interspecific and intraspecific variation in mating calls in female túngara frogs by mapping expression of the activity dependent gene egr-1.
Despite this, FCU has only rarely been used as the phenotypic expression of the activity of uric acid transporters in clinical studies exploring the genetic basis of hyperuricaemia.
Testosterone inhibits the expression of the activity of lipoprotein lipase, the main enzymatic regulator of triglyceride uptake in the fat cell, preferentially in abdominal fat (28, 29).
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(D) The effects of miR-27b expression on the activity of wild-type and mutant 3′UTRs of NR2F2 were analyzed by the dual luciferase reporter assay.
Thus, the effect of cellodextrin transporter expression on the activity of the cell surface cellulase was negligible.
Moreover, no difference between genotypes was observed in the expression of the activity-regulated cytoskeleton-associated protein (Arc), an immediate early gene whose expression is a marker of increased neuronal activity (data not shown).
Since certain level of Tor activity was essential for the expression of FLO11, the activity of Tor should be maintained in this range.
Thus, to define the medium composition and the induction conditions of a process for the soluble expression of rPly, the activity is the main response to be considered.
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Justyna Jupowicz-Kozak
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