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Different cell types pose different challenges, ranging from engineering immune acceptance to ensuring expression of sufficient levels of insulin and of insulin secretion in response to glucose.
Vernalization refers to the expression of sufficient [Vrn1] to enable the promotion of Δ[Vrn3].
Vernalization saturation is the expression of sufficient Vrn1 and this will eventually occur in all facultative wheat plants due to low base expression.
One of the key bottlenecks in studying transmembrane proteins has been expression of sufficient amounts of protein for biochemical and structural characterization.
This group demonstrated that expression of sufficient Bcl-2 to neutralize the Bim is critical in effector/memory T-cell survival.
It remains to be investigated, why the native Arabidopsis ProDH genes do not enable the expression of sufficient amounts of active and correctly targeted protein, whereas the Arabidopsis P5CDH gene produced a functional protein with its native mTP [ 19].
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The study described herein is an expansion to that study, investigating the expression profile of sufficient numbers of blood samples as proposed by Arceo et al. [ 22].
As a result, expression of a sufficient amount of MP to enable structural studies is the main bottleneck in MP structure determination.
Nevertheless, we have validated the stable expression of a sufficient number of miRNAs for each condition, which the user can confidently use in qPCR analysis.
Expression of quantities sufficient for structural work is one problem, but stability is probably the single most important barrier to progress.
Fur is conserved in a wide variety of bacterial species and functions similarly in all of them by repressing gene expression under conditions of sufficient cellular iron.
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