The quantitative analysis of expression levels of specific products was calculated and statistically evaluated using relative expression software tool from Pfaffl, MW et al. (Pfaffl MW, Horgan GW, Dempfle L. Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR.
Accordingly, we investigated whether knockdown of HLA by RNA interference (RNAi), a ubiquitous regulatory system that can efficiently and selectively inhibit the expression of specific gene products, would enable allogeneic cells to evade immune recognition.
Differences in molecular properties of cells may result from genetic manipulation via gene transfer vectors or, as is the case for cancer, are manifest as a result of inappropriate expression of specific gene products in the diseased tissue.
Transition from maternal to zygotic transcripts is accompanied by expression of specific genes whose products associate with protein binding.
It will be important now to determine if this is a direct genomic effect or if expression of specific HIV gene products is involved.
Our model iRR1083 serves also as a framework to integrate further gene expression and proteomic data and explain the expression level of specific gene products during infection.
The ability to measure the expression of specific proteins that are gene products associated with breast cancer has led to important advances in breast cancer treatment.
These proteins bind to specific sites on DNA and (for example) turn on expression of specific genes.
The concept relies on expression of gene products to generate specific antibodies for protein analysis, such as immunolocalization of the proteins on cellular and subcellular level.
Besides, this methodology can be used, for instance, to simultaneously compare expression of stage-specific gene products, measure distinct cellular pathways, or evaluate the activity of an inducible or treatment-responsive promoter as compared to a constitutive internal viability control, respectively driving the expression of the two luciferases in the same parasite.
