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As gene fragmentation dissociate the expression of selection marker with that of the GOI, additional cloning and screening steps are necessary for selection of high producing cell clones.
Furthermore, the presence of internal promoters required to drive the expression of selection cassettes can influence the expression of flanking genes.
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Up to now, with the exception of the above mentioned studies on gene expression, evidences of selection acting on GC content per se have been scant (see [ 3] for review).
The expression of a selection of differentially expressed genes was examined by QPCR of individual cells.
We further validated the stable expression of a selection of those constitutively expressed candidates in the different stress conditions by SYBR Green qPCR.
The differential expression of a selection of the genes identified as being differentially expressed was validated by applying real-time quantitative RT-PCR (qRT-PCR).
Because research on natural selection assessments has found that the format of the question plays an important role in students' expression of natural selection concepts and misconceptions (Nehm and Schonfeld 2008), we also used one of the open-response questions from the ACORNS instrument (Nehm et al. 2012).
EPO yield and substrate-specific productivity of the recombinant BHK-21 cells have been compared to control cells without the PYC2-gene but transfected with the plasmid coding for the expression of the selection genes and EPO.
We have also validated one of those modules experimentally, showing that over-expression or inhibition of the miRNA assigned as a regulator changes significantly the expression of a selection of module genes, thereby confirming the inference of the algorithm.
There has only been one published study on the transcriptional response of Drosophila to sigma virus infection, which used qPCR to measure the expression of a selection of immunity genes [20].
Lentiviral vectors were constructed with expression cassettes that allowed the expression of antibiotic selection markers (e.g., puromycin) in order to identify and enrich the fraction of transduced cells (Figure 1C).
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