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However, the injection of an unrelated dsRNA did not affect the expression of selected genes differentially expressed after subolesin knockdown.
These results revealed that the expression of selected genes was altered specifically in A6 cells cultured under clinorotation.
Fig. 2 Validation of the expression of selected genes from RNA-Seq using qRT-PCR.
(A) qRT-PCR analysis of the expression of selected genes in IBV infected A549 cells compared with uninfected controls.
The expression of selected genes was validated by RT-qPCR.
Expression of selected genes was determined by using two-step quantitative real-time PCR.
Altered expression of selected genes was validated by qRT-PCR (Figure 4c).
Differential expression of selected genes was further confirmed by RT-PCR (Table 3).
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Differential expression of selected genes in both gene arrays and qRT-PCR are presented in Table 6.
Differential expression of selected genes was confirmed using quantitative real-time PCR.
Expression of selected genes was determined by reverse transcription quantitative real time PCR (RT-qPCR).
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