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The expression of selected differentially-expressed genes was verified with quantitative (q) RT-PCR (see below).
These data suggest that exposure to hypergravity may interfere with the expression of selected cerebellar proteins.
Such age-dependent differential expression of selected miRNAs might suggest their involvement in ageing-associated changes in cutaneous wound repair.
These results revealed that the expression of selected genes was altered specifically in A6 cells cultured under clinorotation.
Steroid hormone concentrations were analyzed for various tissues, as well as expression of selected steroidogenic genes.
Furthermore, also the abrogation of CNOT2 expression via CRISPR-Cas9 resulted in increased expression of selected MHC II genes (Supplementary Fig. 6).
After tamoxifen-driven CNOT3 deletion, naïve macrophages and T cells were analyzed for expression of selected MHC II genes by RT-qPCR.
Only a limited number of functional studies has been conducted on P. oceanica, mostly looking at the expression of selected target genes e.g., refs 7,8,9,10.
As we have shown in this study, RollFISH can also be used to screen the expression of selected biomarkers across hundreds of tumor samples in parallel on TMAs.
Fig. 2 Validation of the expression of selected genes from RNA-Seq using qRT-PCR.
(A) qRT-PCR analysis of the expression of selected genes in IBV infected A549 cells compared with uninfected controls.
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