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Rumen papillae were collected on d 24 and 28 to quantify mRNA expression of select genes.
We confirmed the differential expression of select genes by qRT-PCR and showed an overall concordance of relative expression levels with those obtained by microarray analysis (Figure 3A and 3B).
Quantitative real-time PCR (qRT-PCR) analysis was used to verify the relative expression of select genes, with preference given to either known HNF4α targets [21], or genes that contained promoters previously indicated in the literature to bind HNF4α in human cells [22], [62].
Description of data: Technical validation of gene expression of select genes.
The gene expression of select genes was validated by quantitative real-time PCR.
However, the magnitude and timing of expression of select genes within these pathways differs between studies.
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However, the injection of an unrelated dsRNA did not affect the expression of selected genes differentially expressed after subolesin knockdown.
Furthermore, the expression of selected genes shown to regulate or be expressed by NSCs is not different by RT-PCR or by microarray.
These results revealed that the expression of selected genes was altered specifically in A6 cells cultured under clinorotation.
Fig. 2 Validation of the expression of selected genes from RNA-Seq using qRT-PCR.
(A) qRT-PCR analysis of the expression of selected genes in IBV infected A549 cells compared with uninfected controls.
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