It is interesting that, in the HCC tissues, the expression of raft-associated mTOR was significantly increased, but the amounts of phosphorylated BAD were significantly reduced in the lipid rafts, without changing the total amount of raft-associated BAD.
Additionally, each fraction was tested for expression of lipid raft and nonraft markers Flotillin-1 and transferrin receptor (TfR), respectively, using immuno-dot blot.
Additionally, given the strong expression of TRAF2 in the raft microdomain of NOD Th40 cells (Figure 4), the possibility for interaction of induced cFLIPp43 with TRAF2 and further activation of Nf-κB, as has been demonstrated [34], arises.
Expression of palmitoylation-impaired (raft-excluded) CD44 mutants in non-invasive MCF-10a cells was sufficient to reversibly induce the phenotypic appearance of epithelial-to-mesenchymal transition and to increase cell motility.
Fas expression in lipid raft and non-raft fractions was then investigated.
These studies indicate that inhibition of c-FLIP can enhance the expression of Fas in lipid raft regions.
Overall, we demonstrated that the HDAC inhibitor MS-275 sensitizes OS cells to FasL-induced cell death by increasing the expression of Fas in lipid raft microdomains, which correlates with c-FLIP downregulation.
Mass spectrometric analysis of lipid rafts of AC showed the expression of 5-LO, whereas lipid rafts of LC did not.
On RAFT TEs, expression of corneal epithelial differentiation markers, such as cytokeratins 3/12, is typically only observed in superficial cell layers following culture at the air/liquid interface which triggers spontaneous stratification [23].
Overall, our studies indicate that MS-275 sensitizes OS cells to FasL by upregulating the expression of Fas in membrane lipid rafts, which correlates with the c-FLIP-dependent distribution of Fas to lipid rafts.
The increased abundance of miR-200c showing 67% more expression in rafts than in monolayers (P<0.08) by array analysis was verified by northern blotting.
