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To validate whether the up-regulated or down-regulated genes identified by statistical analysis were involved in SGIV infection, we detected the relative expression of partial genes using quantitative real time-PCR (qRT-PCR).
The present data from qRT-PCR analysis validated the hypothesis that expression of partial genes is regulated by SGIV infection, including cytokine, cytokine receptor and transcription factor, apoptosis-associated genes, interferon-related genes, and cytoskeleton genes.
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Moreover, they analyzed the expression patterns of partial genes and discussed the relationship between differentially expressed genes and TPD [ 21].
Therefore, these partial PLK copies appear to be pseudogenes, or remnants of partial gene duplications, based on: i) lack of expression evidence, ii) apparent gene chimerism or rearrangements, iii) truncated length, and/or iv) premature stop codons.
These products may result from the expression of a partial gene.
This approach also enables simulation of gene over-expressions and partial gene knockdowns in addition to gene knockouts.
Moreover, 77% of the partial genes that might be considered as pseudogenes exhibit evidence of an RNAseq expression.
We suspect that clumping was a function of partial expression of the gene.
Taken together, our transcriptome sequencing analysis suggests that the concurrence of heat and drought stress will not only alter expression profiles of partial individual stress responsive genes but also trigger activation or depression of a proportion of HD specific genes, leading to a complicated gene regulatory network in wheat acclimation response to drought and heat combination.
Results revealed a remarkable pattern of partial and differential expression of conotoxin genes among and within species and that species exhibit a variety of expression patterns including over-dispersed and under-dispersed expression of gene families.
However, in many species, dosage compensation is partial and the expression of many genes is not compensated [ 8].
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