Sentence examples for expression of nub from inspiring English sources

This correlates well with reported expression of nub transcripts in adults [ 42].

This aberrant immune gene expression was effectively blocked by expression of Nub from a transgene.

The elevated AMP gene expression was NF-κB/Relish-dependent, and could be blocked by transgene expression of Nub-PD, demonstrating its capacity to directly down-regulate expression of immune defense genes.

Importantly, flies that lack expression of Nub-PD protein have a significantly changed gut microbiome, indicating that the maintenance of a normal gut flora is dependent on negative gene regulation by Nub-PD.

Our gene expression analysis of nub 1 mutants revealed that in the adult gut, there is differential expression of similar clusters of genes that previously have been linked to nervous system development (101 genes), development and differentiation of wing and legs (89 genes), and subgroups of these GO terms (Additional file 8).

In infected flies, over-expression of nub-RD did not reveal a significant effect on the expression of CecA1 and Dipt (data not shown).

However, approximately 10-fold over-expression of nub-RD was reached routinely using leaky expression from the same transgene combination (UAS-nub-RD/hs-Gal4).

It is unclear why this is the case, but it may indicate that strong over-expression of nub-RD results in negative feedback regulation, most likely at the post-transcriptional level.

Flies for over-expression of Nub-PD (w 1118 ; UAS-nubRD) (described below and in Additional file 1) or down-regulation of Nub (UAS-dsnub) (VDRC #6218) [ 54] were crossed with the Gal4 driver lines c564-Gal4 (w 1118 ; P{GawB} c564) (fat body) and hs-Gal4 (w P{w[ +mc]  = Gal4-Hsp70.PB}).

In homozygous nub 1 flies (Additional file 1), expression of the nub-RD transcript is strongly reduced [ 20] and no Nub-PD protein is detected on immunoblots (Additional file 2B), whereas the Nub-PB protein is still expressed in the nub 1 flies, confirming that nub 1 mutation is specifically affecting the expression of the Nub-PD protein.

A possible consequence of the elevated AMP gene expression in guts of nub 1 flies is that the numbers of commensal bacteria would be reduced.