We therefore used induction of alkaline phosphatase (ALP) expression to monitor the effect of different receptor complex arrangements.
A separate hierarchical clustering was therefore performed for these 37 early genes, and the levels of expression were monitored at later phases of the disease.
To examine the constitutive and developmental expression of OsGLP genes, expression was monitored using gene-specific primers for all 12 genes in three positions of non-stressed leaves in three cultivars: Azucena, IR64, and SHZ-2 (Table 4).
The level of Akt expression was monitored using an antibody that recognizes Akt irrespective of its phosphorylation state.
Knockdown of eIF2Bε expression was monitored by Western blot analyses (Fig. 13A).
The total level of BAD expression was monitored by blotting with an anti-BAD antibody (lower panel).
Knockdown of ARL6 expression was monitored by immunoblotting with anti-TbARL6 antibody.
Restoration of TAP-2 expression was monitored by ICC by using a specific antibody for TAP-2 (AMS Biotechnology, Milton Park, Milton, UK).
After 72 hours the cells were extracted and the levels of PP1γ expression monitored by western blotting.
Studies by Lu et al [4] demonstrated the feasibility and utility of monitoring the expression of miRNAs in human cancer tissue.
