MicroRNAs are endogenously expressed ∼22 nucleotide RNAs, which downregulate the expression of large numbers of target genes in vivo [14], [15].
This approach requires prior data on gene expression of large number of genes, generally determined by microarray technology or expressed sequence tags (ESTs).
However, the possibility of side effects, due to the expression of large amounts of angiogenic factors, justifies the design of devices that express synergistic molecules in low controlled doses.
It helps determine how a cell can control the expression of large numbers of genes simultaneously.
Expression of large tumour-associated glycoproteins in non-transformed mammary cells promoted focal adhesion assembly and facilitated integrin-dependent growth factor signalling to support cell growth and survival.
Gene expression of large and small subunits of arsenite oxidase respectively showed 15- and 17-fold higher expression.
The rapid construction of a BAC-based expression vector facilitates heterologous expression of large gene clusters for drug discovery.
Using a "DNA chip", which measures the expression of large numbers of genes simultaneously, researchers identified a signature of 51 genes, allowing TB to be distinguished from both latent TB and other diseases that mimic the disease's symptoms.
Additionally, biochemical and structural studies of membrane proteins require expression of large amounts of the protein in bacterial cells, and these studies are hampered by the deleterious effects of expression on cell growth.
Because SCOOP minimises costs for cloning and expression screening, it constitutes an interesting alternative for establishing expression of large gene collections.
We believe the proposed method has a number of potential applications in the representation and expression of large objects with three-dimensional laser scanner data.
