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Since Moore et al. (Moore et al., 2004) only assessed the position of efnb-expressing cells in the neural plate without assessing their expression of eye field markers, it is unclear whether this is also the case in Xenopus.
In the present investigation the effect of expression of eye cell lines [rabbit corneal epithelial cell line (SIRC)] on highly porous HAp scaffolds were compared with the normal fibroblastic cells on HAp in general, in terms of in vitro dissolution studies, cell culture cytotoxicity and cell adhesion properties.
This Rx3-regulated gene network supports a hierarchical expression of eye field transcription factors specifying early eye development.
We also evaluated the levels of expression of eye field transcriptional factors and the expression of genes associated with the RPE phenotype (mitf and tyr).
Indeed, ephb4a-expressing transplants located in the telencephalon and abutting the edge of the eye field maintain expression of eye field markers in at least some of the cells of the transplant (Fig. 5E,F), while some efnb2a-expressing transplants located in the eye field do not show expression of eye field markers (Fig. 5G,H).
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Analyses to the specific genes, including Tyr, Ush2a, Edar, Oca2, Mpv17, Rom1b, Skp1, and differential expression of eye-related genes, transcriptional factors and circadian rhythm pathway genes, and similarity cluster of the Tlr gene family and crystallins.
We have previously shown, by ChIP sequencing of Ab targets and expression array analysis, that Ab regulates the expression of eye-antennal cell fate genes and that this effect is enhanced or altered in ab scrib − tumours (Turkel et al., 2013).
A survey of spatiotemporal distributions of Vmem during craniofacial development revealed two spots of hyperpolarized cells (lighter stain) that become eyes; artificial depolarization of these cells by misexpression of a cation channel altered expression of endogenous eye markers and induced eye defects.
Hence, the Wnt-PCP pathway appears to be directly implicated in eye fate determination because it controls the expression of specific eye field genes.
Since co-expression of ab with RhoGEF2, Src64B or DRac1 also affects expression of the eye differentiation factor, Elav, we sought to determine whether other cell fate genes in eye and antennal development were also affected in these tumours.
The expression of the eye field transcription factor Rx starts at around E7.5 in the anterior neural plate [30].
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