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Further investigation should be carried out to establish transformed insect cell lines with stable expression of corresponding interfering RNAs.
The expression of corresponding miRNA target genes was measured using TransScript II Green One-Step qRT-PCR SuperMix (Transgen) with beta-actin and GAPDH as internal controls.
NATs can affect the expression of corresponding sense genes in cis or in trans at multiple levels (Pelechano and Steinmetz, 2013).
The result of dual-luciferase reporter assay and qRT-PCR showed that alncRNAs could inhibit transcriptional activity of TFs and expression of corresponding microRNAs.
While ExMV-derived mRNAs may be translated in target cells into appropriate proteins, miRNAs regulate expression of corresponding mRNA species, and both RNA-depended ExMV-mediated mechanisms lead to functional changes in the target cells.
Many lncRNAs are involved in gene expression regulation with neighboring genes as targets, so the expression of corresponding neighboring genes (ZEB1 vs. ZEB1-AS1; PTENP1-AS1 vs. PTENP1, DICER1 vs. DICER1-AS1, MORC2 vs. TUG1, HOXC11 vs. HOTAIR) was examined.
However, regardless of higher silique carbohydrate content in HOCL, it is not certain that expression of corresponding carbohydrate related genes are really higher than that in LOCL during rapeseed seed development.
Next, we measured the expression of corresponding chemokines in prostates during regrowth, to determine a mechanism for MSC homing.
Analysis of the proteome using 1D-PAGE coupled with LC-MS/MS resulted in the detection of 41 proteins for which expression of corresponding transcripts was not detected by microarray.
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Up- or down-expression of corresponding genes in related lines was confirmed by RT-PCR.
In the first dataset, cells transformed to malignancy by mutant p53 and activated Ras are perturbed with the aim of restoring gene expression to levels found in non-transformed parental cells via retrovirus-mediated re-expression of corresponding cDNAs or shRNA-dependent stable knock-down.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com