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Additional file 3: Differential gene expression of controls and cisplatin-treated MCF7 cells.
COXI immunolabelling showed mosaic COXI expression in cultures A C, with numerous cells exhibiting reduced COXI, unlike the homogenous expression of controls (Fig. 3Ba d).
Flow cytometry revealed a significant decrease to less than 30% of the expression of controls after 96 h of incubation (P<0.0001, n=3).
To investigate alterations in gene expression of controls and exposed fish, we used two independent analytical methods, MA plots (M, the intensity ratio, versus A, the average intensity for a dot in the plot) and normal quantile (q-q) plots, as described in detail in Supplemental Material (available online at http://www.ehponline.org/members/2008/11627/suppl.pdf).org/members/2008/11627/suppl.pdf
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Data are expressed as mean ± SEM (units are relative to the expression of control group).
One-way observation is in some ways an expression of control.
Whether consciously or not, some healthy eaters' healthy eating is primarily an expression of control, cleanliness and virtue.
a Transient expression of control, showing that the expression of the GFP protein was distributed throughout the cell.
No difference in the expression of control genes was found in WS cases versus normal controls, although this is another potential source of variation.
MRP mRNA expression of control (C1) cells was 80%, EpRas13 (E13) clone was 33% and EpRas14 (E14) clone was 20% of parental EpRas cells (Fig. 2a and b).
Protein expression data are presented as a percentage of mean expression of control group ± SE.
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