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In parallel, media samples were taken to track expression of construct 4 by PAGE and western blot (Fig. 5A, B).
Cells were incubated for 48 96 hours, as indicated, to allow expression of construct.
PDE activities were then normalised for expression of construct, and the data were normalised to untreated (NT) wild type PDE4D7 activity.
Still, the LI was unable to support expression by itself in the absence of the enhancer elements of the promoter, as demonstrated by the low expression of construct MP+I.
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Expression of constructs expressing the PBRs of both Rac1 and RhoA tagged to GFP showed that the PBRs of both proteins functioned as NLSs, with the Rac1 PBR being a stronger NLS [35].
Transgenic mice carrying these constructs were made and expression of the construct was monitored by staining for β-galactosidase activity.
The expression of constructs was examined by fluorescence and confocal microscopy, immunohistochemistry, and β-galactosidase activity assays.
Transient expression of constructs encoding AACT1 and AACT2 fused to GFP revealed that the two proteins show a different subcellular localization.
Vectors for checking the expression of constructs in mammalian cells and for harboring the foreign genes as DNA vaccine are suggested.
Expression of constructs was confirmed by reverse transcriptase PCR (RT-PCR).
Expression of constructs encoding both the DMPK 11-15 minigene and GFP-DMPK 3'UTR with the expanded CTG repeats resulted in nuclear foci and aberrant RNA splicing.
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