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In figure 5B, the expression of ASMA (red) and smoothelin (green) can be observed.
Results showed increased expression of ASMA and CALP in vitro [ 72].
Positive expression of ASMA, calponin, MHC, SM2 and SMemb was strong and diffuse within the cytoplasm.
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Expression levels of ASMA, calponin and SM2 significantly differed between vessels, except between mPA and either bPA, Ao and IVC vessels.
The primary cardiac fibroblasts used in this study exhibited basal mRNA and protein expression of the contractile protein ASMA, however, ASMA stress fibers were not detected in control cells by immunocytochemistry (data not shown).
Quantitative real-time PCR analysis revealed that TGFβ induction of ASMA gene expression was significantly reduced on stretched cells.
CD68, CD34 and ASMA expression of freshly isolated FBR CD68+ cells compared to that of 1 week and 3 weeks cultures is summarized in figure 4. 93.6±2.7% of the cells isolated showed to be positive for the CD68 antigen, whereas 8.2±6.6% of these freshly isolated cells were CD34+ and no ASMA+ cells were present.
BCFs and NTFs were characterized by their expression of the mesenchymal marker, ASMA, and absence of the epithelial marker, CK19.
The combined effects of mechanical stretch and TGFβ treatment on human primary cardiac fibroblast expression of the differentiation marker ASMA and the pro-fibrotic markers collagen 1 and 3 were investigated.
Fibroblast differentiation to the more active myofibroblast form is a hallmark of cardiac fibrosis, and is associated with increased collagen production, enhanced proliferative and migratory potential, and is associated with increased expression of alpha-smooth muscle actin (ASMA) [ 9].
Part of Asma Begum's face, one of her arms and her chest were burnt.Some of the victims of acid attacks die of severe burns.
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