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Expression of a selected set of 20 genes were monitored and validated through real-time PCR for specific induction during early and late stage of interaction in three rice genotypes.
To analyze the in vitro response to IFNß at baseline we measured the expression of a selected set of three known IFNß response genes and IFNß itself in resting and IFNß treated PBMCs by quantitative realtime PCR.
To directly examine tissue expression of a selected gene, we performed whole mount in situ hybridization in the 96 hours post fertilization zebrafish using a probe designed to detect MS4A17A.17 RNA.
The host of other TFs in that cell type may either be involved in fine-tuning gene expression or in regulating the expression of a selected subset of genes.
From data presented in this manuscript, it is obvious that treatment with increasing doses of AA induces a specific down regulation of expression of a selected set of genes, resulting in an arrest of cell proliferation and, at higher doses, in cell death.
We have also experimentally verified the expression of a selected set of known and novel miRNAs.
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To identify genes under the early control of CRL1, we monitored the expression kinetics of a selected subset of genes, mainly chosen among those exhibiting differential expression, in crl1 and WT following exogenous auxin treatment.
Reverse transcription (RT) quantitative real-time PCR (qPCR) is the most accurate and easy-to-perform technique to measure the expression level of a selected gene of interest by quantifying mRNA transcripts.
Another approach is to select genes highly correlated with ESR1 and define molecular subtypes corresponding to pathological ER status based on the bimodal distribution of the expression levels of a selected gene set[10], [11].
The stable expression level of a selected set of conserved miRNAs (miR159, miR167 and miR168) in diverse plant sectors across successive growing phases (Figure S1) strongly supports this idea.
Expression patterns of a selected set of DON- and ZEA-induced genes were validated by the use of quantitative RT-PCR after exposure to the toxins.
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