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At 6 hpi (cluster 3) a further three members of this pathway showed peak expression (CD14, EIF2AK2 and MYD88) and at 24 hpi (cluster 4) peak expression of a further two genes (IRAK4 and TLR6) was observed.
Crucially, addition of DMOG in combination with EGF significantly increased expression of a further 11 genes (in addition to the 9 genes upregulated in response to either DMOG alone or hypoxia alone).
The expression of a further 27 proteins was compared in serum-starved HMLECs by immunoblotting with specific antibodies, using enhanced chemiluminescence for detection, and densitometry for quantification (QuantityOne, BioRad).
We also examined the expression of a further 191 phosphatases in the TCGA database and used principal component and comparative marker analysis available from the Broad Institute to recapitulate the TCGA-defined subgroups and identify the specific phosphatases defining each subgroup.
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In total using Passel, Human Protein Atlas and UniProt, we found evidence of protein expression for a further 54 (2.7%) of the 2001 genes in the potential non-coding set (for more details, see Supplementary Material and Supplementary Material, Fig. S9).
The expression of survivin, a further anti-apoptotic and Hsp90 client protein, in drug-treated cells was higher than those in control samples.
Like each episode of Strolling, it functions as both a refreshing fountain of personal expression and a further layer of connective tissue in Emeke's ambitious diasporic project.
As shown in Figure 2, these biomarkers featured different patterns of expression suggesting a further sub classification with regard to their response in pre-tumor and tumor state.
Interestingly, three tumours in which no mutation was detected showed complete absence of expression and a further nine showed chimeric staining pattern with both positive and negative areas.
Functional expression of a gene can further be regulated by a multitude of post-translational mechanisms.
Since a source of expression data in UC is unavailable for some of the genes of interest, it will be important to demonstrate an inverse relationship between hypermethylation and expression of a gene before further investigation of function in UC.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com