Exact(7)
The ability to manipulate genes through cloning and expression methodologies have allowed for the development of novel chimeric receptors to selectively target cancer when introduced into immune cells.
The reasons for this discrepancy probably reflect differences in protein expression methodologies and the enhanced sensitivity of SPR to detect protein protein interactions.
Affinity-based enrichment distinguished cells are based on specific antigen expression methodologies, where the target cells are either labeled with a fluorescent/magnetic bead tag or directly captured on a stationary substrate.
Genetic engineering of the C. reinhardtii chloroplast represents a promising strategy to address the issues of cost, scalability and adaptability faced by current expression methodologies, but only if robust recombinant protein expression can be achieved.
The success of previous two-transcript diagnostics shows that, despite being formulated using microarray platforms, these intrinsically simple classifiers can be implemented efficiently through pre-existing gene expression methodologies.
Both studies point to the potential of gene expression methodologies to refine the accuracy of clinical prognosis for patients undergoing resection for primary LAC especially in early disease stages.
Similar(53)
Cell free expression methodology is well suited for pharmaceutical protein expression and engineering and will probably become more commonly used in the future.
These results are consistent with previous observation using transient expression methodology (Fig. 1, 2), suggested that stable overexpression of Large induced the functional glycans independent to Gal, sialic acids, and complex N-linked glycans.
The introduction of cloning expression methodology [4], [5] has led to the successful cloning of a glycosyltransferase and to the demonstration that overexpression of a glycosyltransferase cDNA clone can confer the capability of glycan biosynthesis in overexpressing cells [6].
Although MMP-8 and elastase-2 will require further prospective validation studies for confirmation, the results are a relevant demonstration that our gene expression methodology can successfully be leveraged against the tremendous heterogeneity in patients with SSAKI.
On the other hand, to what extent these differences arise from overexpression of TPC isoforms in different host cells, the expression methodology employed, or the method by which NAADP is introduced into the cell awaits clarification by way of future studies.
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