Q-RT-PCR was used to measure the mRNA expression levels of various differentially expressed genes between adjacent normal and lung tumors by using 384-well plates (ABI PRISM 7900 HT Sequence Detection System).
cDNA microarrays were done to determine and compare the native expression levels of various cell cycle proteins expressed in G0-G1, S, G2-M phases and other upstream molecules, in both glioma and their treatment with hUCBSC.
To determine whether MEK1DD- and MEK2DD-expressing cells undergo an EMT, we examined the localization and measured the expression levels of various epithelial and mesenchymal markers.
Fig. 3 Brain mRNA expression levels of various apoptosis-related factors evaluated by real-time PCR and expressed as fold changes relative to the control group.
The effects of AgNPs on the expression levels of various intracellular proteins in HePG-2 cells were detected by Western blotting as previously reported [36, 37].
We investigated expression levels of various inflammatory cytokines on mRNA level using RT-PCR arrays and qRT-PCR; furthermore expression at protein level was studied using immunohistochemistry.
Experimental Design: The expression levels of various antiangiogenic factors in four glioma cell lines were evaluated after chronic in vitro treatment with temozolomide by Western blot.
Here, we show that emodin treatment caused G2/M arrest in liver cancer cells and increased the expression levels of various genes both in mRNA and protein level.
Another EV study has shown that the expression levels of various proteins within vesicles are enhanced in a mouse model of NAFLD, and that protein expression pattern differs between exosomes and microvesicles (Povero et al., 2014).
Using the reverse transcription PCR, we evaluated expression levels of various antigen presentation-related genes, including LMP2, LMP7, MECL-1, PA28α, PA28β, TAP1, TAP2, and tapasin, in two oral squamous cell carcinoma cell lines, HSC5 and HSC7.
The mRNA expression levels of various pro-inflammatory mediators such as cytokines, adhesion molecules, chemokine, and matrix metalloproteinase were analyzed by quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR).
