The expression levels of selected differentially expressed genes were normalized to endogenous glyceraldehyde-3-phosphate dehydrogenase expression level and calculated with the 2-ΔΔ Ct formula (ABI Prism 777 Sequence Detection System User Bulletin no. 2).
Expression levels of selected miRNAs in gastric cancer tissues were expressed as fold-change over adjacent normal tissues using the DDCt calculation (DDCt 1/4 DCt, tumor-DCt, adjacent normal) [ 14].
d, Expression levels of selected markers in the t-SNE space.
We examined the expression levels of selected genes that either encode ECM structural components or are involved in ECM remodeling across the five models (Fig. 2).
To further study the relationship between candidate prognostic and predictive biomarkers in the tumor microenvironment, we focused on the RNA expression levels of selected genes using the TCGA dataset.
b, Expression levels of selected marker genes for all tumour-associated CD3+ T cells from patient 7, including those unable to be resolved as CD4 or CD8 (ND, not determined) based on scRNA-seq (Supplementary Table 10).
The expression levels of selected genes were also analyzed both in experimental group and in vivo blastocysts recovered from uteri as control group.
To further substantiate this view, we compared the expression levels of selected genes that are either positively (rpsV, katE, pdhR, bolA and poxB) or negatively (sdhB) regulated by the σ38 containing RNA polymerase by qRT-PCR in the ∆yeaG mutant and wild-type strains following 24 h of N starvation.
The purpose of this study was to investigate the effects of age on phagocytosis and antibacterial activity of peripheral blood neutrophils, and on expression levels of selected mRNA specific for neutrophil migration or killing functions.
We measured the expression levels of selected genes involved in cell cycle.
Expression levels of selected miRNAs detected by qRT-PCR were normalized to miR-39 and analyzed using the 2−ΔΔCT method.
