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Additionally, endogenous and exogenous auxin positively modulated the expression levels of multiple abiotic stress-related genes (RAB18, RD22, RD29A, RD29B, DREB2A, and DREB2B), and positively affected reactive oxygen species (ROS) metabolism and underlying antioxidant enzyme activities.
Gene expression signatures can predict the activation of oncogenic pathways and other phenotypes of interest via quantitative models that combine the expression levels of multiple genes.
QKI is known to regulate RNA expression levels of multiple genes and we reasoned that the silencing experiments should modify mRNA expression patterns in the cells.
One important feature of CSCs is their resistance to the cytotoxicity of varous chemotherapeutic agents because of their relatively high expression levels of multiple drug resistance transporters such as ABCB1 (MDR1/P-glycoproteinotein) and ABCG2 (MXR/BCRP) [38].
Specifically, this method could allow the accurate determination of the expression levels of multiple transcripts that could lead to the false positive of an expression differential by hybridization due to the high identity at nucleotide level.
For example, in yeast, the TOR and the PKA pathways sense the source and availability of carbon and nitrogen and regulate the expression levels of multiple gene groups [1] [3].
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Previously, we reported our success in improving n-butanol tolerance in E. coli by engineering an Artificial Transcription Factor (ATF) that can modify the expression level of multiple targets simultaneously and improved the n-butanol tolerance of MG1655 strain to 1.5% (vol/vol) n-butanol.
Changes in the expression level of multiple proteins within a pathway increase the confidence that the differential expression of a single protein using traditional statistical methods is likely to be associated with the studied condition [19].
The integrative approach using random small RNA libraries and cell-based genetic selection provides a novel technology platform for identifying RNA effectors capable of modifying complex cellular phenotypes through control of the expression level of multiple targets.
This is a relevant finding made possible by the QRT-PCR assessment of the expression level of multiple ETS genes in cancer and normal prostate tissue samples, which to our knowledge had not been evaluated before.
Expression level of multiple genes in a given orthogroup was averaged.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com