In contrast to Figure 2A and 2B, in dnFGFR1 versus dnFGFR4 injected embryos we see that expression levels of relatively few probe sets are greater than two-fold different between the two groups (Figure 2C).
The expression levels of a few miRNA families, such as MIR156 and MIR166, were obviously high in three libraries.
Semi-quantitative reverse transcriptase PCR analysis showed differences in the expression levels of a few genes in parental lines.
The CAI and codon usage models are originally based on somewhat qualitative assumptions about the expression levels of relatively few genes.
There are other methods such as mRNA differential display and RNA fingerprinting by arbitrary primed PCR but both of these methods are unsuitable for experiments where the expression levels of relatively few of the genes are expected to vary [ 10- 13].
While Sorek et al.'s finding that reducing the expression levels of a few toxic genes increases their transferability to E. coli supports our hypothesis about the impact of expression level on HGT, our hypothesis goes well beyond the mechanism of cytotoxicity and the small number of toxic genes.
To further confirm the expression levels of a few selected genes, we performed semi-quantitative real-time PCR using the same RNA samples isolated from lymphoid cells that had been exposed to the various pathogenic agents/vehicle and used to carry out the microarray experiments.
The expression levels of only a few matrix proteins (nacrein, pearlin, MSI60, aspein, prismalin-14, and MSI31) at different larval development stages have been analyzed by RT-PCR and are related to shell formation in P. fucata [ 21].
However, the functional analysis of HNF4α regulated-APR genes so far has mainly relied on the description of the expression level of a few selected genes [ 21].
The relative expression level of a few known miRNA families, such as miR159, miR167, miR160, miR165 and miR390, were similar to that of Arabidopsis[ 44] (Table 2).
