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Periplasmic expression level of the mutants was also improved.
It is evident that the expression level of the control gene actin was indistinguishable (Fig. 3a).
This high expression level of the protein in soluble form improves its applicability for industry.
The expression level of the ACTIN2 gene (AT3G18780) was used as a loading control.
The expression level of the rice Actin gene was amplified as a control.
qPCR was applied to further validate the expression level of the above 7 down-regulated genes.
The expression level of the nine reference genes with Ct value ranged from 14.22 to 25.66.
This result may be explained by the differences in expression level of the different targeted molecules.
The expression level of the fusion proteins had no relationship with the linker length.
c GNS4 mRNA expression level of the RNAi and overexpression transgenic plants.
The expression level of the double mutein was augmented significantly and the enantioselectivity remained high.
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