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A SCN slice that had successful YC expression was transferred to the recording chamber and superfused with warmed oxygenated regular ACSF.
At first, protein samples were checked by 12% SDS-PAGE to screen the recombinant strains, and then the sample of highest expression was transferred to nitrocellulose (NC) filter membranes.
Figure 4 shows how the co-expression is transferred from transcript level to protein level in Plasmodium falciparum.
Since co-expression is transferred between the protein and the transcript level, this high fraction of co-expressed genes might be due to an accumulation of synchronizing mechanisms at different levels.
To get an overview, and facilitate the analysis, the marked expressions were transferred to an Excel-file with one column for each of the 21 interviews.
The mutated PRH cDNAs present in the mammalian expression constructs were transferred to the pTrc-HisA bacterial expression vector (see the Experimental section).
For expression with the wt RNA-2 leader (delRNA-2), PacI/AscI fragments containing the entire expression cassette were transferred into similarly digested pBINPLUS [53] to give pBD2G12H, pBD2G12HE and pBD2G12L (Figure 1).
The expression vector was transferred into E. coli BL21 DE3).
To determine the stability of pigment expression cultures were transferred to fresh medium for at least five times in the dark.
Expression vectors were transferred into tobacco pollen grains by particle bombardment with a helium-driven particle accelerator PDS-1000/He PDS-1000/HeHercules, CA, USA) using methods modified from Fu et al. [43].
The cbbM expression cassette was transferred into pRS416 using KpnI and SacI, yielding pUDC098.
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