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In molecular level, FEN1 expression is restricted by FEN1 binding with transcription factor YY1. Upon treatment with cancer drugs, YY1 is released from the FEN1 promoter so that the expression of FEN1 is highly induced in cancer cells, consequently leading to drug resistance.
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However, the extent of HSV-2 MS-GFP spread in the eyes (i.e., area of GFP expression) was restricted by an order of magnitude in 0ΔNLS-immunized mice relative to naïve controls (Fig. 7).
Initially, cell-free expression was restricted by several limitations, especially for the expression of transmembrane proteins or proteins rich in disulfide bonds.
Its expression was restricted by doxycycline treatment to the first 8 hours, 16 hours or 40 hours of a 40-hour total incubation period.
To investigate the proapoptotic effects of the γ-secretase inhibitor GSIXII and to define the range of active concentrations, we treated the breast cancer cell line MDAMB231 with increasing concentrations (4 to 20 μ M) for 48 hours before evaluation of apoptosis by measuring the expression of Apo2.7 antigen (whose expression is restricted to dying cells) by using flow-cytometry analysis.
Note that Ac expression is restricted to the anterior compartment by a mechanism that is independent of Wg [20] and thus dSno clones in the posterior compartment do not express ectopic Ac.
(A ) Dlx5/6-Cre expression is restricted to forebrain, as demonstrated by rosa26 LacZ and mT/mG reporter lines.
syt12Gal4 expression is restricted to secretory cells as showed by UAS-RFP (red).
By 36hpf, expression is restricted to anterior structures, including the forebrain and hindbrain (Fig. 5Biv).
It is well possible that this age-specific difference relies on the fact that inhibition of apoptosis by systemic application of omigapil acts on many cell types, whereas, inhibition of apoptosis by Bcl2 expression is restricted to muscles.
A total of 102 candidate genes were identified that express differentially in the A and P compartments; four were characterized: Stubble (Sb ) expression is restricted to A cells due to repression by en.
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