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This is true whether Bim expression is manipulated by the CDK inhibitor (RCV), Bim-directed shRNAi, or by protein synthesis inhibitor (CHX).
In conclusion, an excellent correlation is observed between Bim expression and apoptosis in cAMP-stimulated IPC cells, whether the Bim expression is manipulated by RCV, CHX or various Bim-directed shRNAi hairpins.
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In studies of breast cancer cell lines and tumors, expression of miR-155 and FOXO3a was inversely correlated and FOXO3a protein expression was manipulated by increasing or inhibiting miR-155 expression [41].
INHBA, the third prominent gene of the signature in addition to COL11A1 and THBS2, is not included in the list, because its expression was manipulated by the transfections with consistent results.
2) The central hypothesis of the paper (Yan fluctuations are functionally relevant) can be rigorously tested only if the noisiness of Yan expression is manipulated independently from EGF signaling, e.g. by manipulating transcriptional of translational control elements of Yan.
More studies in which Bim expression is manipulated are necessary to support such a conclusion.
RasGRF1 expression was manipulated in RA FLS by cDNA transfection and gene silencing, and effects on MMP-1, TIMP-1, MMP-3, IL-6, and IL-8 production measured by ELISA.
Hence, we generated transgenic rice plants, where MIPS gene expression was manipulated tissue specifically through the use of rice Ole18 promoter, by an RNAi mediated approach.
EpCAM expression was manipulated in breast cancer cell lines using RNA interference and cDNA expression constructs.
Everything is manipulated by the party.
BMP activity is manipulated by LDN-193189 (Stemgent Inc).
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