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The expression is from the title of William Prosser's article on products liability, The Assault upon the Citadel (Strict Liability and the Consumer), 69 Yale L.J. 1099 19600).
Using cycloheximide, a classic inhibitor of protein translation, we first clarified what portion of dsDNA-induced VCAM1 expression is from direct sensing of dsDNA and what part is from secondary indirect activation, requiring de novo protein synthesis.
Its peak expression is from phase G2 to cytokinesis.
The onset of Rx3 expression is from ~9 hpf, during the late gastrulation stage [ 19].
Differential gene expression is from [23] in standard rich conditions (YPD medium).
However, most of the expression is from neither endosymbionts nor E. coli (Additional file 3: Figure S3).
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The majority of transcript expression was from two cathepsin L genes on chromosome 10 (LOC659441 and LOC659502).
For cathepsin B, the major expression was from genes on chromosome 3 (LOC663145 and LOC663117).
The pRL-TK vector driving Renilla luciferase expression was from Promega.
Plasmids for gRNA cloning and hCas9 expression were from Addgene (Cambridge, Massachusetts).
In all cases the favorable allele increasing constitutive expression was from the tropical japonica parental line (Moroberekan and Azucena).
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