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These observations are consistent with the relaxed fidelity observed for the second-generation nitroTyr-RSs and the near perfect fidelity observed for the first-generation nitroTyr-RSs (when expression is conducted in the absence of nitroTyr).
Immunohistochemical (IHC) staining for MLH1, MSH2, MSH6, PMS2 expression is conducted on all surgically resected CRC and EC pathologic specimens per institutional standard, and all patients receive a hand-out describing universal MMR screening procedures that includes a toll-free number for the Department of Clinical Genetics.
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Rubisco expression were conducted by 0.2 mmol/L IPTG for 6 h at 22°C.
The analysis of diurnal gene expression was conducted as described in Filichkin, Breton et al. (2011b).
Optimization of Vip3A16 protein expression was conducted using Plackett Burman design and response surface methodology.
Methods: Characterization of tumor cell lines regarding hA3R protein expression was conducted using Western Blot, flow cytometry and immunofluorescent staining.
Comparative analysis of synthetase gene expression was conducted between the initial and shuffled strains using FQ (fluorescent quantitation) RT-PCR (reverse transcription polymerase chain reaction).
A synthetic consensus gene was designed based on residues of the amino acid sequences of dengue envelope domain III (scEDIII) from all four serotypes, and codon optimization for expression was conducted using baker's yeast, Saccharomyces cerevisiae.
The initial screen for transgene expression was conducted on pectoral muscle samples.
IHC of fibroblast surface protein expression was conducted on tumor sections to identify the first TAF characteristic.
Such a comparison in Fst expression was conducted separately in males and females (n = 3×20 flies per line).
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